mouse hrp cd68 sc 20060 santa cruz  (Santa Cruz Biotechnology)

Journal: iScience

Article Title: IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4 + T cells

doi: 10.1016/j.isci.2023.107464

Figure Lengend Snippet: IL-27Rα deficiency halts the protective effect of the SPY1 vaccine (A) Mice were intranasally immunized with SPY1 plus CT or PBS plus CT weekly for 1 month. On day 28, mice were intranasally challenged with S. pneumoniae strain 19F and specimens were collected for analysis on day 31. (B). Bacterial colonization in nasal lavage fluid of each group after 19F challenge (n = 5–7). One-way ANOVA. Mean ± SEM.∗p < 0.05, ∗∗p < 0.01. (C) One week following the last immunization, mice were challenged intranasally with S. pneumoniae strain D39 (5 × 10 7 CFU). The survival rates were observed for 21 days post challenge (n = 10). Log rank test.∗p < 0.05. (D) H&E and peroxidase immune staining for Ly6G (neutrophil marker) and CD68 (macrophage marker) in the indicated groups 3 days after 19F challenge. Representative slides are shown for each group (200 ×; scale bars = 100 μm). (E) Total inflammation score. One-way ANOVA. Mean ± SEM.∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (F) Quantification of neutrophils surrounding bronchioles from (D). One-way ANOVA. Mean ± SEM.∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (G) Quantification of macrophages surrounding bronchioles from (D). One-way ANOVA. Mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: Tissue sections were also stained with polyclonal goat anti-mouse CD68 and Ly6G (servicebio) and detected using a streptavidin biotin immuno-peroxidase system.

Techniques: Staining, Marker

Journal: iScience

Article Title: IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4 + T cells

doi: 10.1016/j.isci.2023.107464

Figure Lengend Snippet: The immune protective effect of SPY1 vaccine is independent of glycolysis (A) Titers of sIgA in saliva and IgG in serum (n = 7–8). T-test. Mean ± SEM. (B) H&E staining and peroxidase immune staining in lung tissues for Ly6G and CD68 in WT SPY1 vaccinated mice that were administered either PBS (control) or 2-deoxy-glucose (2-DG) 3 days after 19F challenge. Representative slides are shown for each group (200 ×, scale bars = 100 μm). Total inflammation score and quantification of neutrophils and macrophages surrounding bronchioles (n = 3–6). T-test. Mean ± SEM. (C) ELISA to quantify IL-17A protein levels in lung tissues (n = 6–7). T-test. Mean ± SEM. (D) Representative flow cytometric plots depicting proportions of Th17 cells in the indicated groups. (E) The proportions and numbers of Th17 cells in for the indicated groups determined by flow cytometry (n = 5). T-test. Mean ± SEM.

Article Snippet: Tissue sections were also stained with polyclonal goat anti-mouse CD68 and Ly6G (servicebio) and detected using a streptavidin biotin immuno-peroxidase system.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: iScience

Article Title: IL-27 mediates immune response of pneumococcal vaccine SPY1 through Th17 and memory CD4 + T cells

doi: 10.1016/j.isci.2023.107464

Figure Lengend Snippet:

Article Snippet: Tissue sections were also stained with polyclonal goat anti-mouse CD68 and Ly6G (servicebio) and detected using a streptavidin biotin immuno-peroxidase system.

Techniques: Purification, Recombinant, Lysis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Acta Neuropathologica Communications

Article Title: Microglial CD68 and L-ferritin upregulation in response to phosphorylated-TDP-43 pathology in the amyotrophic lateral sclerosis brain

doi: 10.1186/s40478-023-01561-6

Figure Lengend Snippet: Immunohistochemistry panels for analysis of microglial changes in the human ALS and hTDP-43-ΔNLS ‘rNLS’ mouse brains

Article Snippet: Mouse IgG 3 anti-CD68 (1:200; Abcam, Ab783) , Goat anti-mouse IgG 3 AlexaFluor® 594 (1:500; ThermoFisher, A21155) , Rabbit anti-GFAP (1:2,000; DAKO, Z0334) , Goat anti-rabbit AlexaFluor® 594 (1:500; Jackson Laboratories, 111–586-144).

Techniques: Immunohistochemistry